Determination of Residues of 26 β2-Agonists in Pork Liver by Reversed Phase High Performance Liquid Chromatography-Tandem Mass Spectrometry
10.11895/j.issn.0253-3820.140457
- VernacularTitle:反相液相色谱-串联质谱法检测猪肝中26种β2-受体激动剂类药物残留
- Author:
Jia LIU
;
Yunfeng XIE
;
Dandan REN
;
Xingpin CUI
;
Guirong LIANG
;
Yongtan YANG
- Publication Type:Journal Article
- Keywords:
β2-Agonist;
High performance liquid chromatography-tandem mass spectrometry;
Pork liver;
Residues
- From:
Chinese Journal of Analytical Chemistry
2014;(10):1486-1492
- CountryChina
- Language:Chinese
-
Abstract:
A method for determination of residues of 26 β2-agonists in pork liver was developed using high performance liquid chromatography with tandem mass spectrometric ( HPLC-MS/MS ) . After enzymatic hydrolysis with β-Glucuronidase/Arylsulfatase for 12 hours, the pH of sample solution was adjusted to 1 using perchloric acid for protein precipitation. The precipitate was extracted with 0. 1mol/L perchloric acid aqueous. The extracts in the above two steps were combined and adjusted to pH 4 for the solid phase extraction ( MCX) . And then the 26 β2-agonists residues in the extracts were separated on a reversed phase HPLC column using a gradient elution program of 0. 1% formic acid aqueous solution (A) and 0. 1% formic acid in acetonitrile solution ( B) . Multiple reaction monitoring ( MRM) with positive polarity was selected to monitor qualitative and quantitative ion. Based on the optimized method, 26 β2-agonists could be analyzed in 15 min. The recoveries ranged from 64 . 0% to 112 . 7% for the 26 kinds ofβ2-agonists residues with three spiked levels of 5, 10 and 20 μg/kg. The relative standard deviations ( RSDs) were less than 15. 2%. The limits of detection (LOD) for the 26 kinds of β2-agonists were 0. 15-1. 35 μg/kg.
