Effect of Combined Hypoxia and High Fat Diet on eNOS/NO of Myocardium in Experimental Rats
10.3969/j.issn.1000-3614.2014.09.017
- VernacularTitle:低氧联合高脂饮食对SD大鼠心肌内皮型一氧化氮合酶/一氧化氮的影响
- Author:
Yanxia ZHAO
;
Yuhong LI
;
Yaping WANG
;
Yingzhong YANG
;
Lan MA
;
Rili GE
- Publication Type:Journal Article
- Keywords:
Hypoxia;
High fat diet;
Endothelial nitric oxide synthase;
Nitric oxide
- From:
Chinese Circulation Journal
2014;(9):723-727
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To investigate the effect of combined hypoxia and high fat diet (HFD) on endothelial nitric oxide synthase (eNOS)/nitric oxide (NO) of myocardium in experimental rats with its possible mechanisms.
Methods: A total of 60 male SD rats were randomly divided into 3 groups, n=20 in each group. Control group, the rats were fed by normal diet with normal oxygen condition. Hypoxia group, the rats were fed by normal diet with simulated 5000m altitude oxygen condition. Combined hypoxia and HFD (H+HFD) group, the rats were fed by HFD and simulated 5000m altitude oxygen condition. All animals were treated for 4 weeks and peripheral blood and myocardium specimen were collected. Hemoglobin was examined by automatic blood cell analyzer, plasma malondialdehyde (MDA) was measured by TBA method, superoxide dismutase (SOD) activity was detected by WST-1 method, mRNA and protein expressions of eNOS were examined by real time PCR and Western blot analysis respectively, the myocardium nitrates and nitrites (NOx) was measured by nitrate reductase method.
Results: Compared with Control group, Hypoxia group and H+HFD group had increased mRNA and protein expressions of eNOS, H+HFD group had lower NOx levels than the other 2 groups P<0.05. Compared with Hypoxia group, H+HFD group showed obviously increased total cholesterol, LDL-cholesterol and decreased SOD activity, diseased MDA level P<0.05.
Conclusion: Upon hypoxia alone, H+HFD may further reduce NOx level of myocardium, it implies aggravated chronic hypoxia impairment, which might be related to dyslipidemia and lack of anti-oxidative ability in experimental rats.
