Simultaneous HPLC Determination of Hesperidin, Magnolol, Honokiol and Liquiritin in Soft Capsule Jia-Wei Huo-Xiang Zheng-Qi

VernacularTitle:高效液相色谱法同时测定加味藿香正气软胶囊中橙皮苷、厚朴酚、和厚朴酚和甘草苷的含量
Author: Yanfei CAO ; Zhenzhong WANG ; Yuan BI ; Zhengkuan WANG ; Yaozhong LYU ; Qin WAN ; Wei XIAO
Publication Type:Journal Article
Keywords: Soft Capsule Jia-Wei Huo-Xiang Zheng-Qi; magnolol; honokiol; liquiritin; hesperidin; HPLC
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(8):1768-1771
CountryChina
Language:Chinese
Abstract: This study was aimed to develop an HPLC method for the determination of hesperidin,magnolol,honoki-oland liquiritin in Soft Capsule Jia-Wei Huo-Xiang Zheng-Qi (JWHXZQ). AKromasil C18 column (250 mmí4.6 mm, 5 μm) was used with water-methanol as mobile phasegradient elution. The flow rate was 1.0 mL·min-1, and the de-tecting wavelength was at 287 nm. The results showed that the linearity ranges ofhesperidin,magnolol,honokioland liquiritinwere 4.47-178.70 μg·mL-1, 3.42-136.96 μg·mL-1, 3.49-139.48 μg·mL-1, 3.92-157.20 μg·mL-1, respec-tively (r>0.999). The average recoveries of them were 99.48%, 99.05%, 99.57% and 99.79%, respectively. It was concluded that the method was accurate, sensitive and specific for quality control of Soft Capsule JWHXZQ.