Study on Preventing Efficacy of Combined Using of Three Classical Tibetan Prescriptions in Focal Cerebral Ischemia Injury of Rats
10.11842/wst.2014.08.029
- VernacularTitle:3种藏药经典方联用对大鼠局灶性脑缺血损伤的保护作用
- Author:
Bin XIE
;
Ruiying LIU
;
Jingping HE
;
Rui TAN
- Publication Type:Journal Article
- Keywords:
Cerebral ischemia;
MCAO;
Er-Shi-Wu-Wei Shan-Hu pill;
Ru-Yi Zhen-Bao pill;
Er-Shi-Wei Che n-X iang pill
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(8):1846-1849
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to investigate the preventing efficacy of the combined using of Er-Shi-Wu-Wei Shan-Hu (ESWWSH) pill, Ru-Yi Zhen-Bao (RYZB) pill, Er-Shi-Wei Chen-Xiang (ESWCX) pill on the focal cere-bral ischemia injury in rats. A total of 80 Sprague-Dawley (SD) rats were randomly divided into four groups. In the sham operation group and the MCAO model group, normal saline was given orally every day (1 mL/100 g). In the positive control group, nimodipine was given orally every day (1.89 mg/100 g). In the drug combination group, ESWWSH pill (7 mg/100 g), RYZB pill (31.5 mg/100 g), ESWCX pill (49 mg/100 g), were separately given in the morning, noon and evening every day. Improved MCAO was used in the model establishment after 7-day intragastri-cal administration. After 24 h brain infarction, TTC method was used in the determination of the cerebral infarct size. Drying and weighing method was used in the calculation of brain water content. Determinations were made on con-tent of NO in ischemic brain issues (nitrate reductase method); activities of SOD in serum (xanthine oxidase method);content of MDA in serum (TBA method); and activities of LDH in serum (pyruvate method). The results showed that compared with the MCAO model group, the combined using of three Tibetan classical prescriptions can obviously re-duce the cerebral infarct size, brain water content and the NO content in brain issues, increase the activity of SOD, reduce the content of MDA, and reduce the activity of LDH in serum. It was concluded that the research preliminari-ly showed that the combined using of ESWWSH pill, RYZB pill and ESWCX pill can protect the ischemic brain tis-sues, such as reducing the cerebral infarct size, alleviating brain edema, and inhibiting oxidative stress reactions.
