Identification of Polygalae Radix and Its Adulterants by psbA-trnH Sequence

VernacularTitle:远志药材及其混伪品的DNA条形码鉴定
Author: Xiaoxi MA ; Weichao REN ; Wei SUN ; Yuan TU ; Yaqin ZHANG ; Ming SONG ; Junlin YU ; Bin LI ; Shilin CHEN
Publication Type:Journal Article
Keywords: Polygalae radix; psbA-trnH sequences; DNA Barcoding; identification
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(8):1719-1724
CountryChina
Language:Chinese
Abstract: In this study,Polygalae radix and its adulterants were identified by psbA-trnH sequence.The genomic DNA was extracted from forty-six samples, the psbA-trnH sequences were amplified and sequenced Bi-directionally, and then assembled sequences by Codoncode Aligner V 3.7.1. The genetic distances were computed by kimura 2-parameter (K2P) model, and the Neighbor-Joining tree was constructed by MEGA 6.0. Results showed that minimum intra-specific K2P distance of Polygala tenuifolia and Polygala sibirica were 0.004 and 0, which were smaller than the maximum intra-specific K2P. The NJ tree showed Polygalae radix can be distinguished from its adulterants by psbA-trnH sequences. Therefore, using psbA-trnH sequences can distinguish Polygalae radix from its adulterants.