Identification of Pinelliae Rhizoma and Its Adulterants Based On ITS2 Sequence

VernacularTitle:中药材半夏及其混伪品的DNA条形码鉴定研究
Author: Yaqin ZHANG ; Ming SONG ; Wei SUN ; Li XIANG ; Xiaoxi MA ; Jiajia FAN ; Jun WANG ; Xiaocun ZHANG ; Xia LIU
Publication Type:Journal Article
Keywords: Pinelliae Rhizoma; DNA Barcoding; ITS2 sequence; adulterants
From: World Science and Technology-Modernization of Traditional Chinese Medicine 2014;(8):1725-1729
CountryChina
Language:Chinese
Abstract: In this study, the ITS2 sequence was used to identify Pinelliae Rhizoma and its adulterants to ensure its market circulation, clinical effect and safety. All genomic DNA from 59 samples were extracted successfully. The Kimura 2-Parameter (K2P) distances and NJ tree were calculated using software MEGA 6.0. The length of the ITS2 sequence of Pinelliae Rhizoma was 251 bp. The intraspecific genetic distance was smaller than the interspecific ones;The NJ tree indicated that Pinelliae Rhizoma distinguished from its adulterants obviously. The results showed that the ITS2 sequence can be used to distinguish Pinelliae Rhizoma from its adulterants accurately and stably.