Determination of Content and Related Substances of Piperaquine in Artemisinin and Piperaquine Tablets by HPLC
10.11842/wst.2014.08.011
- VernacularTitle:HPLC法测定青蒿素哌喹片中哌喹的含量及相关物质
- Author:
Bin QIN
;
Guo YIN
;
Yan YAN
;
Zhenhao DING
;
Yuan LI
;
Tiejie WANG
- Publication Type:Journal Article
- Keywords:
A rtemisininand Piperaquine Tablets;
content determination;
related substances;
HPLC
- From:
World Science and Technology-Modernization of Traditional Chinese Medicine
2014;(8):1747-1753
- CountryChina
- Language:Chinese
-
Abstract:
This study was aimed to establish determination method of content and related substances of piperaquine in A rtemisinin and Piperaquine Tablets, and to set the limit of related substance. HPLC was adopted on a SHISEIDO CAPCELL PAK C18 (4.6 mm í 250 mm, 5 μm) using an isocratic mobile phase consisted of acetonitrile: 0.1%trichloroaceticacid:triethylamine (18:82:0.2, V:V:V, pH 2.5) with a flow rate of 1.0 mL·min-1. The column tempera-ture was kept at 30oC and the detection wavelength was set at 216 and 237 nm, separately for the determination of related substance and content. The results showed that piperaquine and its related impurity can be separated effec-tively. The concentration-response relationship was linear over the range of 0.01-0.2 mg·mL-1 (R2=0.999 9). The av-erage recovery rate was 98.14% (RSD=0.77%, n=9). The minimum detection limit was 0.06 μg·mL-1. The solution was stable for 12 h. It was concluded that the method was specific, accurate, sensitive and suitable for the determi-nation of content and related substances of piperaquine in A rtemisinin and Piperaquine Tablets.
