The effects of Cyr6 1 on the biological behavior of human adenoid cystic cancer cells

VernacularTitle:Cyr61对人腺样囊性癌细胞系生物学特性的影响
Author: Ruizhi ZHANG ; Ping ZHANG ; Bo YU ; Rui LUO ; Zhenglin GONG
Publication Type:Journal Article
Keywords: Adenoid cystic cancer; Cyr6 1; Proliferation; Invasion; Apoptosis
From: Journal of Practical Stomatology 2014;(4):526-529
CountryChina
Language:Chinese
Abstract: Objective:To explore the effects of cysteine-rich 6 1 (Cyr6 1 )on biological behavior of human adenoid cystic carcinoma ACC-LM and ACC2 cells.Methods:The chemically synthesized Cyr6 1-siRNA was transfected into ACC-LM and ACC2 cells.Cell proliferation was measured by the MTT method,the invasive ability was evaluated by Transwell chamber assay,and cell apoptosis was analyzed using flow cytometry by double staining with Annexin V and propidium iodide.Results:Cyr61-siRNA significantly down-regu-lated Cyr61 protein expression in ACC-LMand ACC2 cells.Cyr61-siRNA markedly inhibited the proliferation and invasion of the cells, however,there was no significant difference in cell apoptosis between Cyr6 1-siRNA and control groups.Conclusion:Cyr6 1 promote the proliferation and invasion of adenoid cystic cancer cells.