Role of connective tissue growth factor-integrin β1 signal pathway in the proliferation and migration of pulmonary arterial smooth muscle cell
10.3760/cma.j.issn.2095-428X.2014.13.014
- VernacularTitle:结缔组织生长因子-整合素β1受体信号途径在肺动脉平滑肌细胞增殖和迁移中的作用
- Author:
Jie FU
;
Cuixia LU
;
Gang LI
;
Yu HU
;
Peng JIA
;
Jian ZHAO
;
Bin LIU
- Publication Type:Journal Article
- Keywords:
Integrin;
Connective tissue growth factoial;
Pulmonary arterial smooth muscle cell;
Proliferation;
Migration
- From:
Chinese Journal of Applied Clinical Pediatrics
2014;29(13):1005-1009
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the mechanisms of integrin β1 on connective tissue growth factor(CTGF)-induced proliferation,migration,change of cytoskeleton of pulmonary arterial smooth muscle cell(PASMC) in vitro,and to investigate the effects of CTGF-integrin β1 signal pathway on pulmonary vascular remodeling in pulmonary arterial hypertension (PAH).Methods Pulmonary artery smooth muscle cells of SD rats were cultured in vitro.WST-1 assay was used to detect the effects of anti-integrin β1 antibody on CTGF-induced proliferation of PASMC.Transwell chambers were used to observe the effects of anti-integrin β1 antibody on CTGF-induced migration of PASMC.The cytoskeletal rearrangement was observed with coomassie brilliant blue R250 staining and Confocal Lasar Scanning Microscopy (CLSM).Results Different concentration of anti-integrin β1 antibody could inhibit the proliferation of PASMC induced by CTGF,which presents concentration dependent pattern (P ＜ 0.05).The higher the concentration of anti-integrin β1 antibody,the more severity the proliferation of PASMC induced by CTGF was inhibited.and inhibition rate of PASMC proliferation was the highest at 72 hours.Anti-integrin β1 antibody(15 mg/L) decreased significantly the number of PASMC passing through Transwell induced by CTGF,compared with CTGF group (P ＜ 0.01).Meanwhile,antiintegrin β1 antibody could change cytoskeletal rearrangement of PASMC induced by CTGF.Conclusions Integrin β1mediates the proliferation,migration,cytoskeletal rearrangement of PASMC induced by CTGF.The CTGF-integrin β1signal pathway may play a key role in proliferation,migration,cytoskeletal rearrangement PASMC.
