Expression of miR-218 in cervical cancer tissues and bioinformatics analysis of predicted target genes
10.3760/cma.j.issn.1006-9801.2014.07.002
- VernacularTitle:miR-218在子宫颈癌组织中的表达及预测的靶基因的生物信息学分析
- Author:
Xiaoxia HU
;
Kangkang ZENG
;
Xianglan MO
;
Fei LIU
;
Wenjing ZHENG
- Publication Type:Journal Article
- Keywords:
Uterine cervical neoplasms;
miRNA-218;
Oligonucleotide array sequence analysis;
Target gene;
Bioinformatics
- From:
Cancer Research and Clinic
2014;26(7):437-441
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of miR-218 in cervical cancer tissues and bioinformatically analyze the target genes of miR-218 to provide theoretical basis on further studies of miR-218 functions in cervical cancer.Methods miRNA array was applied to detect miRNA expression profile in cervical cancer tissues,and real-time RT-PCR was used for validation.The bioinformatic analysis of the target genes of miR-218 involved gene ontology and signal transduction pathway enrichment was performed.Results miR-218 expression significantly decreased in cervical cancer tissues compared with that of normal cervical tissues.The functions of predicted target genes of miR-218 were enriched in biological regulation,adhesion and motion,proteometabolism,cellular differentiation,protein binding and other biological processes and molecular functions.According KEGG pathway database,the predicted target genes involved in pathway in cancer,adherent junction,focal adhesion,prostate cancer,chronic myeloid leukemia,melanoma,and other signal transduction pathways.Conclusions miR-218 expression significantly decreases in cervical cancer tissues.Some of the predicted target genes of miR-218 are significantly enriched in tumor related with signaling pathways.
