The relationship between expression of caveolin-1 and epithelial-mesenchymal transition and metastasis in pancreatic cancer
10.3760/cma.j.issn.1007-631X.2014.07.018
- VernacularTitle:Caveolin-1与胰腺癌上皮间质转化及转移关系的研究
- Author:
Chen HUANG
;
Kundong ZHANG
;
Weidong WU
;
Tao JIANG
;
Jun CAO
;
Zhengjun QIU
- Publication Type:Journal Article
- Keywords:
Pancreatic neoplasms;
Caveolin 1;
Neoplasm metastasis;
Epithelia-mesenchymal transition
- From:
Chinese Journal of General Surgery
2014;29(7):545-547
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the relationship between the expression of Caveolin-1 (Cav-1) and epithelial-mesenchymal transition(EMT) or metastasis in human pancreatic cancer.Methods The Cav-1 protein and EMT markers in highly metastatic pancreatic cancer cell lines (L3.7,Panc02-H7) and poorly metastatic pancreatic cancer cell lines (COLO357,Panc02) was detected by Western blotting and immunofluorescence.The morphology of the pancreatic cancer cells were observed under phase-contrast photomicrographs.The plasmids pcDNA3.1-caveolin-1 and control vector pcDNA3.1 were transfected into COLO357 cells by Lipofectamine LTX.The expression of Cav-1 protein,E-cadherin and vimentin were detected,and the morphology of COLO357 cells observed.Results L3.7 and Panc02-H7 cells had strong positive Cav-1 staining and high expression of mesenchymal marker (vimentin,N-cadherin) and low epithelial marker (E-cadherin,β-catenin),whereas COLO357 and Panc02 cells with typical epithelial morphology were weak positive in Cav-1 staining and of low expression of vimentin,N-cadherin and high expression of E-cadherin,β-catenin.In Cav-1 transfected COLO357 cells vimentin expression increased,and E-cadherins decreased resulting in typical morphology changes of EMT.Conclusions Overexpression of caveolin-1 is correlated with epithelial-mesenchymal transition of pancreatic cancer cells and cancer metastasis.
