Genotyping and drug resistance analysis of multiple drug resistant Escherichia coli
10.3969/j.issn.1673-4130.2014.18.023
- VernacularTitle:多重耐药大肠埃希菌的基因分型及耐药分析
- Author:
Jianhong HUANG
;
Jinwen HE
;
Xin LIN
;
Caiyun XUAN
;
Lin HUANG
;
Yongcong ZHAN
;
Jielin TIAN
- Publication Type:Journal Article
- Keywords:
Escherichia coli;
genotype;
drug resistance
- From:
International Journal of Laboratory Medicine
2014;(18):2476-2477
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the genotype ,distribution and drug resistance of 24 multi-drug resistant Escherichia coli i-solates from the patients in the hospital 24 cases of multi-drug resistant genotyping and resistance ,the distribution of its depart-ments in the hospital ,to provide basis for rational use of antibiotics in clinical patients .Methods By MIC method to analyze antimi-crobial susceptibility of Escherichia coli ,Enterobacter Genetics adopt consistent between repetitive sequences (ERIC)-polymerase chain reaction (PCR) screening carried out the multi-drug resistant Escherichia coli genotyping .Results Statistics showed that the hospital were isolated 24 strains were divided into two types A and B2 type genotype .Distribution is 10 in respiratory ,6 in urology , 5 in ICU (B2 type 3) ,3 in hematology and oncology (B2 type 1) .Drug susceptibility results of 4 type B2 ,for cefoxitin ,ceftazidime , amikacin ,ticarcillin + clavulanic acid are all manifestations of resistance .24 strains of Escherichia coli ,amoxicillin ,cephalothin , gentamicin ,ticarcillin ,piperacillin resistance rate reached more than 70% .Conclusion B2-type strain showed multidrug resistance , pathogenicity strong ,and mainly in the ICU and hematology and oncology ,the use of antibiotics should be based on reasonable ad-justments in patients infected with different genotypes of Escherichia coli .