The suppression of IL-24 on pancreatic cancer mediated by tumor-targeting oncolytic adenovirus in immune-competent mice
10.3760/cma.j.issn.1007-8118.2014.06.017
- VernacularTitle:靶向溶瘤腺病毒介导的IL-24对有免疫功能小鼠胰腺癌移植瘤生长的抑制
- Author:
Bin XU
;
Mingping QIAN
;
Tingsong YANG
;
Zhenshun SONG
;
Xinyuan LIU
;
Bo ZHOU
- Publication Type:Journal Article
- Keywords:
Pancreatic Cancer;
Oncolytic Adenovirus;
Interleukin-24;
T Lymphocyte;
Cytokines
- From:
Chinese Journal of Hepatobiliary Surgery
2014;20(6):457-460
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether IL-24 can inhibit the growth of pancreatic cancer in immune-competent mice,which could be mediated by tumor-targeting oncolytic adenovirus (ZD55-IL-24).Methods Crystal violet staining assay was used to evaluate the anti-proliferative influence of ZD55-IL-24 on Panc02.The tumor growth curve,the tumor formation curve,TUNEL assay,cytotoxic T lymphocyte (CTL) killing assay and serum cytokines detection were used to assess anti-tumor efficacy of ZD55-IL-24 for pancreatic cancer in immune-competent mice.Results ZD55-IL-24 significantly inhibited the growth of pancreatic cancer cells in vitro.Lower tumor formation and slower tumor growth were identified in ZD55-IL-24 group,compared with ZD55-EGFP group or PBS group.ZD55-IL-24 induced pancreatic cancer cells'apoptosis,and mediated anti-cancer immunity by increasing killing responses of CTL to cancer cells and up-regulating serum γ-IFN and IL-6 levels.Conclusion ZD55-IL-24 can induce anti-tumor immunity and suppress pancreatic cancer growth in immune-competent mice.
