Study of macrophage uptake drug and oxygen loaded lipid microbubbles in vitro
10.3760/cma.j.issn.1004-4477.2014.05.017
- VernacularTitle:携氧载药脂质微泡体外巨噬细胞化初步研究
- Author:
Li LIU
;
Shufang CHANG
;
Jiangchuan SUN
;
Mingyue YIN
;
Shenyin ZHU
;
Yi ZHU
;
Zhigang WANG
- Publication Type:Journal Article
- Keywords:
Microbubbles;
Macrophages;
Phagocytosis
- From:
Chinese Journal of Ultrasonography
2014;23(5):434-437
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the feasibility and parameters for macrophage uptake oxygen and paclitaxel loaded lipid microbubbles (OPLMBs) in vitro.Methods OPLMBs were synthesized by mechanical vibration method and incubated with macrophages in different conditions in vitro.The ratio of macrophages uptake OPLMBs was observed by bright field microscope.Results The ratio of macrophages uptake OPLMBs was closely related to the size,concentration as well as the incubation time of OPLMBs with the macrophage.The ratio of macrophages uptake OPLMBs had a reverse relationship with the size of microbubbles.The highest rate of macrophages uptake OPLMBs was (18.9 ± 0.69)％ when the ratio of macrophages to microbubbles was 1 to 10,the time for macrophages co-incubation with microbubbles was 30 min,which was the suitable condition for macrophages uptake OPLMBs.Conclusions OPLMBs can be phagocytized successfully by macrophages in vitro.The size,concentration as well as the incubation time of OPLMBs with the macrophages are important parameters contributed to the efficiency of macrophage phagocytosis.
