Changes of endogenous growth factors and their significance after sciatic nerve injury in rabbits
10.3760/cma.j.issn.1001-8050.2014.05.025
- VernacularTitle:兔坐骨神经损伤后内源性生长因子含量的变化及意义
- Author:
Xiaolei SUN
;
Bin ZHAO
;
Xinlong MA
;
Xiulan LI
;
Kang XU
;
Yang ZHANG
;
Yue GUO
- Publication Type:Journal Article
- Keywords:
Sciatic nerve;
Stem cell transplantation;
Growth factors
- From:
Chinese Journal of Trauma
2014;30(5):471-474
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate variations in bFGF,NGF,and brain-derived neurotrophic factor(BDNF) following sciatic nerve injury in rabbits and the optimum time to perform stem cell transplantation.Methods Sixteen New Zealand rabbits were divided into control group and groups at 3,7,and 14 days postinjury according to the random number table,with 4 rabbits per group.Rabbit models of the sciatic nerve injury were induced by forceps.Structural change of the injured nerve tissues were observed with HE staining.Contents of NGF,bFGF,and BDNF in supernatants of homogenated sciatic nerves were detected by ELISA test.Results Level of bFGF increased slowly postinjury,reached the peak at day 7 (P ＜ 0.05),and then restored to the normal level at day 14 (P ＞ 0.05).Level of BDNF raised quickly postinjury,reached the peak at day 7 (P ＜ 0.05),and then restored to the normal level at day 14 (P ＞ 0.05).Level of NGF increased rapidly postinjury,reached the peak at day 3 (P ＜ 0.05),and then restored to the normal level at day 7 (P ＞ 0.05).Conclusions In the early recovery process after peripheral nerve injury,the nerve tissues regulate the secretion of NGF,bFGF,and BDNF immediately and secretions of these growth factors correlate with the time of injury.Early period (3-7 days) after injury is the best time to perform nerve repairing,nerve transplantation,and stem cell transplantation.
