Expression and clinical significance of Wnt-5a gene in primary hepatocellular carcinomas
10.3760/cma.j.issn.1008-1372.2014.05.004
- VernacularTitle:Wnt-5a在原发性肝细胞癌发生中的表达及临床意义
- Author:
Peifeng LI
;
Xiaohong LIU
;
Yongcheng CAO
;
Cuicui WANG
;
Luting ZHOU
;
Ming GENG
- Publication Type:Journal Article
- Keywords:
Carcinoma,hepatocellular/genetics;
Proto-oncogenes;
Polymerase chain reaction;
Immunohistochemistry
- From:
Journal of Chinese Physician
2014;16(5):588-591
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the expression of Wnt-5a gene in primary hepatocellular carcinoma (HCC) and to expose its role and clinical significance in the development of HCC.Methods Real time quantitative reverse transcription polymerase chain reaction (RT-PCR) was performed in 26 fresh HCC samples and the corresponding para-carcinoma tissues to detect mRNA expression of Wnt-5a gene.Wnt-5a protein was detected with immunohistochemical method in paraffin embedding tissues of 85 cases of HCCs and the corresponding para-carcinoma tissues,and 15 cases of hepatic cirrhosis.Results RT-PCR analysis showed that Wnt-5a mRNA (0.102 127 ±0.158 620) in the HCC tissues was more than that (0.020 106 ±0.022 075) in the para-carcinoma tissues (P＜0.05).The positive expression rate of Wnt-5a protein in HCC,para-carcinoma,and hepatic cirrhosis tissues were 21.2％ (18/85),81.26％ (69/85),and 86.7％ (13/15),respectively.The positive rate of Wnt-5a was significantly lower in the HCC than in the para-carcinoma and hepatic cirrhosis tissues (P ＜ 0.01).The expression of Wnt-5a was significantly associated with lower tumor node metastasis (TNM) stages and small alpha fetoproteins (AFP) content of blood serum (P ＜0.05).Conclusions The high expression of Wnt-5a mRNA was found in the gene transcription of HCC,while Wnt-5a protein was absent or low in HCC.It was suggested that the roles of Wnt-5a was interfered at the protein level rather than the transcriptional level in the HCC.
