Comparison of the expressions of vascular endothelial growth factor and microvessel density in small intestine mucous membrane with urea and creatinine elimination in renal failure rat used Shenfushu and atropine treatment
10.3760/cma.j.issn.1008-1372.2014.05.002
- VernacularTitle:中药肾复舒合用阿托品对肾衰大鼠小肠黏膜VEGF和MVD的影响及其与尿素、肌酐清除的关系
- Author:
Zhefeng JIANG
;
Yunsheng JIANG
- Publication Type:Journal Article
- Keywords:
Kidney failure/drug therapy;
Intestinal mucosa;
CHINESE PATENT DRUGS/pharmacology;
Atropine/pharmacology;
Endothelial growth factors;
Urea/metabolism;
Creatinine/metabolism
- From:
Journal of Chinese Physician
2014;16(5):581-583
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the relationship between that vascular endothelial growth factor (VEGF) expression and microvessel density (MVD) distribution were in the small intestine mucous membrane and that urea and creatinine were eliminated from intestine inrenal failure rat used Shenfushu and atropine treatment.Methods Sprague-Dawely (SD) rats with 5/6 kidney resection were randomly divided into Shenfushu treatment group,Shenfushu + atropine treatment group,pathological control group,and sham operation group for the normal controls.Treatment groups were drenched for eight weeks.Before and after treatment,the concentration of blood urea nitrogen (BUN),serum creatinine (Scr),the fecal urea and fecal creatinine were detected; the expression levels of VEGF and MVD in the small intestinal were detected with small intestinal biopsy immunohistochemical method.The relationship between that expression of VEGF and MVD,and that concentration of BUN,Scr,the fecal urea,and fecal creatinine was analyzed.Results VEGF in the Shenfushu treatment group,Shenfushu + atropine treatment group,and pathological control group was (20.72 ± 1.8) pu,(24.32 ± 1.54) pu,and (28.69 ± 1.82) pu,respectively; MVD was (274.27 ± 10.62)/mm2,(332.71 ± 10.96)/mm2,(436.42 ± 13.70)/mm2,respectively.Expressions of small intestinal VEGF and MVD in two treatment groups were increased significantly than the control group.The concentrations of BUN and Scr were significantly decreased with a negative correlation with the expressions of VEGF and MVD.The concentrations of fecal urea nitrogen and fecal creatinine were significantly increased with a positive correlation with the expressions of VEGF and MVD (P ＜0.05).The concentrations of BUN and serum Cr in the Shenfushu + atropine group were significantly changed more than Shenfushu group (P ＜ 0.05).Conclusions Shenshuaikang and atropine could enhance the expression of VEGF in the small intestinal mucosa,increase microvascular density,promote the elimination of urea nitrogen and creatinine from the intestinal tract to decrease the concentrations of BUN and Scr.Those effects were more obvious with two drugs in combination.
