Effect of insulin on tau protein hyperphosphorylation in APPsw cell and its mechanism exploration
10.3760/cma.j.issn.1674-6554.2014.05.002
- VernacularTitle:胰岛素对APPsw细胞内tau蛋白磷酸化的影响及其机制研究
- Author:
Xu WANG
;
Sujie GAO
;
Song YU
;
Yue WANG
- Publication Type:Journal Article
- Keywords:
Insulin;
Tau;
SH-SY5Y cells;
APPsw
- From:
Chinese Journal of Behavioral Medicine and Brain Science
2014;23(5):388-390
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of insulin at different concentrations on tau protein phosphorylation in vitro using an SH-SY5Y cell line overexpressing APPsw gene (APPsw cell).Methods Western blot was used to detect the expression of tau phosphorylation.The coexpression of between p-GSK-3β and p-tau in APPsw cells was detected by double immunofluorescence and confocal laser scanning microscopy.Results Western blot results showed that 1 000 nM insulin treatment decreased the phosphorylation of tau protein at Thr231 and Ser 396 by(68.91 ± 13.55) and (45.53±22.16),respectively,compared with the normal control (P＜ 0.05) in APPsw cells.The confocal microscopic analysis showed that p-tau and p-GSK-3β colocalized predominantly in cell cytoplasm,and there is some interaction between intracellular expression of p-GSK-3β and p-tau.Conclusion Insulin can suppress the tau protein hyperphosphorylation by potentially inhibiting the activity of GSK-3β.
