Determination of hyperoside in Bushen-Yijing wan by Ion-Pair HPLC
10.3760/cma.j.issn.1673-4246.2014.08.014
- VernacularTitle:离子对色谱法测定补肾益精丸中金丝桃苷的含量
- Author:
Zhengjun WANG
;
Ming LYU
- Publication Type:Journal Article
- Keywords:
Hyperoside;
Bushen-Yijing wan;
Ion-Pair HPLC
- From:
International Journal of Traditional Chinese Medicine
2014;(8):723-725
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the determination of Hyperoside in Bushen-Yijing wan. Methods The HPLC system consisted of the Agilent HC-C18(4.6 mm×250 mm, 5μm)column was adopted. The mobile phase was consisted of acetonitrile(10)-HK2PO4(90)(0.85 g/L sodium heptanesulfonate,H3PO4 adjusted pH to 4.8), the flow rate was 1.0 ml/min, the column temperature was 30 ℃, and the UV detector was set at 360 nm. Results The linear response range was 3.0~60.0μg/ml(r=0.999 9). The average recovery of hyperoside was 98.2%, and RSD1.53%. Conclusions The method is simple, rapid, accurate and repeatable. It can be applied in determination of Hyperoside in Bushen-Yijing wan.
