Construction and identification of an asdA mutant attenuated Salmonella ty phimurium
10.3969/cjz.j.issn.1002-2694.2014.07.001
- VernacularTitle:1株 asdA缺陷型减毒鼠伤寒沙门氏菌作为核酸疫苗载体的构建与鉴定
- Author:
Bin YU
;
Yusi LUO
;
Xiao PENG
;
Shuofeng YUAN
;
Hanben NIU
;
Junle QU
;
Ke ZHANG
- Publication Type:Journal Article
- Keywords:
attenuated Salmonella typhimurium;
DNA vaccine vector;
λ RED-mediated recombination method
- From:
Chinese Journal of Zoonoses
2014;(7):663-668
- CountryChina
- Language:Chinese
-
Abstract:
In this study ,a wild type Salmonella typhimurium (S .typhimurium) strain was isolated and identified in Hong Kong (S129) ,then the asdA gene was knocked out and replaced with kanamycin resistant gene in a Salmonella typhi-murium strain S129 using the λ RED-mediated recombination method .The constructed mutant asdAΔS129 was validated by culturing in the presence or absence of 2 ,6-diaminopimelic acid (DAP) growth in vitro and evaluating its virulence in BALB/c mice challenge assay .Therefore ,this study has demonstrated that an asdA mutant Salmonella typhimurium has been success-fully constructed .
