Application and evaluation of PFGE and MLVA subtyping methods on Brucella genotype in Guangdong Province,China
10.3969/cjz.j.issn.1002-2694.2014.07.014
- VernacularTitle:两种技术在广东地区布鲁氏菌菌株分型中的应用与评价
- Author:
Jingdiao CHEN
;
Xingfen YANG
;
Changwen KE
;
Wenjia LIANG
;
Bixia KE
;
Meizhen LIU
;
Hailing TAN
;
Bosheng LI
;
Wanli ZHANG
- Publication Type:Journal Article
- Keywords:
Brucella;
PFGE;
MLVA;
application;
evaluation
- From:
Chinese Journal of Zoonoses
2014;(7):733-738
- CountryChina
- Language:Chinese
-
Abstract:
To compare and evaluate the discriminatory ability and potential value of pulsed field gel electrophoresis (PF-GE) and multiple locus VNTRs analysis (MLVA) on the genotyping of Brucella ,a total of 60 strains of Brucella and three standards (16M ,544A ,1330S) were genotyped simultaneously by PFGE and MLVA .The result indicated that the similarity coefficient among the 63 isolates was from 72 .1-100 .0% by PFGE ,and could distinguish three species of B .melitensis ,B .su-is and B .abortus at the similarity level of 94 .4% .There were 14 clusters and 29 PFGE types identified by PFGE with discrim-inatory index (DI) of 0 .957 5 at the similarity level of 100% ;the similarity coefficient among the 63 isolates was from 16 .9-100 .0% by MLVA ,and could distinguish three species of Brucella at the similarity level of 52 .3% .There were 8 clusters and 47 MLVA types identified by MLVA with discriminatory index (DI) of 0 .985 2 at the similarity level of 100% .It's suggested that PFGE and MLVA could be used to distinguish three species of Brucella in the similarity coefficient of certain ,but could not effectively distinguish the type in the same species .Both of these two methods could be used for Brucella molecular typing , but MLVA is better than PFGE for its relatively higher discriminating ability .
