Analysis and identification of B cell epitopes of the OMP18 from Campy lobacter jejuni

VernacularTitle:空肠弯曲菌主要外膜蛋白 OMP18的B 细胞抗原表位分析及功能鉴定
Author: Hongqiang LOU ; Ye HU ; Lan WANG ; Xiaoyun SHAN ; Xiusheng SHENG ; Suhua GAO
Publication Type:Journal Article
Keywords: Campylobacter jejuni; OMP18; sequence analysis; epitopes analysis and identification
From: Chinese Journal of Zoonoses 2014;(7):739-742
CountryChina
Language:Chinese
Abstract: In this study ,we aimed to understand the sequence characteristics ,transmembrane structures ,line B cell epitopes present in the OMP18 from Campylobacter jejuni ,and provide candidate antigens for the antibody detection and vac-cine development .NCBI/Blast ,TMHMM Server V2 and DNA Star softwares were used for the OMP18 sequence analysis . Based on the ELISA ,the whole bacterial antibody IgG of Campylobacter jejuni was used for the identification of the predicted line B cell epitopes .The OMP18 gene was found conserved in different Campylobacter jejuni strains .The OMP18 was predic-ted to be located on the outer surface of the bacteria .And three line B cell epitopes were determined to be present in the OMP18 protein .As a conclusion ,the OMP18 protein was confirmed to be an important outer membrane protein ;three line B cell epitopes were identified in the OMP18 ,which could be further used for Campylobacter jejuni antibody detection and vaccine development .