Identification of Culex Flavivirus by deep sequencing approach in Xinjiang, China
10.3760/cma.j.issn.0254-5101.2014.07.005
- VernacularTitle:利用深度测序技术发现新疆库蚊黄病毒
- Author:
Ran LIU
;
Yang ZHENG
;
Rongli DANG
;
Guilin ZHANG
;
Xiang SUN
;
Xiaoming LIU
;
Xiaoyan WU
;
Yuchang LI
;
Yinhui YANG
- Publication Type:Journal Article
- Keywords:
Deep sequencing;
Culex Flavivirus;
Arthropod-borne virus;
Xinjiang
- From:
Chinese Journal of Microbiology and Immunology
2014;(7):513-516
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the diversity of mosquito-borne viruses in Xinjiang , China, and to identify mosquitos-borne viruses of medical importance rapidly .Methods The virus-derived RNAs in mosquitos captured in wild were screened and confirmed by using Illumina deep sequencing approach and reverse transcription PCR , respectively .The alignment analysis was performed by using gene sequences from GenBank.Results One hundred and forty-four Culex Flavivirus ( CxFV, Flavivirus genus, Flaviviridae) specific sequences were identified .The overlapping reads were assembled into 7 uncontinuous viral genomic contigs.The gaps between the contigs were further filled by RT-PCR products, which resulted in reconstruc-tion of viral genomic 5′and 3′terminus (687 nt and 411 nt).Phylogenetic analysis showed that the newly identified CxFV belonged to America/Asian genotype , which specifically clustered into a clade with other CxFV strains from China mainland ,sharing 98.2%-99.5%homologies in nucleotide sequences and 99.5%in amino acids sequences among them .Conclusion Illumina deep sequencing approach was successfully applied to arthropod-borne virus surveillance .The recently emerged Culex Flavivirus was detected for the first time in Xinjiang, China.
