Interleukin-22 promotes Th17 cells differentiation through up-regulating IL-6 production by rheuma-toid arthritis synovial fibroblasts
10.3760/cma.j.issn.0254-5101.2014.07.010
- VernacularTitle:IL-22诱导类风湿关节炎患者滑膜成纤维细胞分泌 IL-6促进 Th17细胞分化的研究
- Author:
Anping PENG
;
Xinyi LU
;
Min HE
;
Haibiao LIN
;
Cheng ZHANG
;
Ruiping LIU
;
Junhua ZHUANG
- Publication Type:Journal Article
- Keywords:
Interleukine-22;
Th17 cell differentiation;
Interleukine-6;
Synovial fibroblasts;
Rheumatoid arthritis
- From:Chinese Journal of Microbiology and Immunology
2014;(7):541-545
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of interleukine-22 ( IL-22 ) on the expression of interleukin-6 (IL-6) by rheumatoid arthritis synovial fibroblasts (RASF), and to analyze their association with IL-17+CD4+T (Th17) cells differentiation.Methods RASF were isolated from six patients with rheu-matoid arthritis ( RA) and cultured in vitro.The expression of IL-6 at mRNA and protein levels by RASF were detected by qRT-PCR analysis and ELISA after treatment with different concentrations of IL -22 for dif-ferent periods of time.Anti-IL-22R1 blocking antibody and inhibitor assay were used to analyze the specific receptor and its downstream signaling pathways associated with IL-6 production.IL-22 pre-treated RASF and CD4+T cells were co-cultured for 3 days in the presence or absence of anti-IL-22R1 or anti-IL-6 to measure the percentage of Th 17 cells by flow cytometry .Results The expression of IL-6 by RASF was increased up-on IL-22 stimulation in a dose and time dependent manner (P<0.05), and that was closely related to IL-22R1 and its downstream signaling pathways of p38 and JAK2 (P<0.05).Co-culturing CD4+T cells with RASF and Transwell system indicated that the percentage of Th 17 cells was increased in IL-22 pre-treated group as compared with that in IL-22 untreated group , but it could be down-regulated by either blocking IL-22R1 or IL-6.Conclusion IL-22 promoted the expression of IL-6 by RASF and further enhanced Th 17 dif-ferentiation.Neutralizing IL-22 in synovium of patients with RA might be an effective therapeutic strategy for RA treatment.
