Expression and purification of the soluble envelope protein of Japanese encephalitis virus
10.3760/cma.j.issn.0254-5101.2014.07.011
- VernacularTitle:乙脑病毒包膜蛋白可溶性表达及纯化
- Author:
Xinyu LIU
;
Yongxin YU
;
Xu LU
;
Ningpan YU
;
Yuhua LI
- Publication Type:Journal Article
- Keywords:
Japanese encephalitis virus;
Envelope protein;
Soluble protein expression;
Purification
- From:Chinese Journal of Microbiology and Immunology
2014;(7):546-550
- CountryChina
- Language:Chinese
-
Abstract:
Objective To express and purify the envelope ( E) protein of Japanese encephalitis virus (JEV) in soluble form.Methods Various prokaryotic expression vectors , host strains and induction conditions including time and temperatures were screened to obtain an optimum prokaryotic expression system for JEV E protein in soluble form .The expressed protein was purified by using nickel column chromatography and gel filtration chromatography .Results The soluble JEV E protein , accounting for 23% of the totally bacteria soluble protein was effectively expressed by using the recombinant plasmid PBCX -E406 at low tem-perature.The purity of the expressed protein reached up to 85%after the purification by using nickel column and gel filtration chromatography .Conclusion Soluble JEV E protein was successfully expressed and puri-fied in a simple and efficient way .It would provide a useful tool for further investigation on JEV infection , attenuation mechanism of JE live vaccine strain SA 14-14-2 and the quality control of JE vaccine .It can also be used for the development of diagnosis assay for JEV .
