Effects of dihydroartemisinin on the expression level of Alpha-7 .3 giardin mRNA in C2 Giardia lamblia

VernacularTitle:双氢青蒿素对 C2株蓝氏贾第鞭毛虫Alpha-7.3 giardin 基因 mRNA 表达水平的影响
Author: Yuan YU ; Yang CHEN ; Shuang GE ; Yang WANG ; Weiwei LI ; Lina ZHAO ; Aqian LIU ; Zhigiang LIN ; Xue GAO ; Xifeng TIAN
Publication Type:Journal Article
Keywords: C2 Giardia lamblia; dihydroartemisinin; Alpha-7.3 giardin; real-time reverse transcription PCR
From: Chinese Journal of Zoonoses 2014;(7):669-672
CountryChina
Language:Chinese
Abstract: Effects of dihydroartemisinin (DHA) on the expression level of Alpha-7 .3 giardin mRNA in C2 Giardia lam-blia was investigated in this study to explore the damage to skeleton protein of C 2 Giardia lamblia .Giardia lamblia was culti-vated respectively for 2 ,4 ,8 ,and 12 hours with modified TYI-S-33 medium containing 100 μg/mL and 200 μg/mL DHA , while the control group performed in the same experimental conditions without DHA .The expressive quantity of Alpha-7 .3 gi-ardin mRNA was determined by using real-time reverse transcription PCR ,and then we found that the expressive quantities of Alpha-7 .3 giardin mRNA with DHA were significantly lower than those in the control group .It’s suggested that dihydroarte-misinin has obvious inhibitory effect on the expression level of Alpha-7 .3 giardin mRNA in C2 Giardia lamblia .The actions of dihydroartemisinin on skeleton protein of C2 Giardia lamblia are effective .