MutS homolog 2 plays a role in Vγ9δ2 T cell-mediated anti-gastric carcinoma immunity
10.3760/cma.j.issn.0254-5101.2014.07.007
- VernacularTitle:hMSH2参与Vγ9δ2T细胞介导的胃癌细胞株杀伤作用
- Author:
Yumei DAI
;
Hui CHEN
;
Chen MO
;
Jingfei SHI
;
Yunfeng LIU
;
Wenli LI
;
Lianxian CUI
;
Wei HE
- Publication Type:Journal Article
- Keywords:
Human MutS homolog 2;
Vγ9δ2 T cells;
Gastric carcinoma;
Innate immunity
- From:
Chinese Journal of Microbiology and Immunology
2014;(7):521-526
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the role of human MutS homolog 2 (hMSH2), a newly identified protein ligand that was recognized by Vγ9δ2 T cells , in innate anti-gastric carcinoma immunity .Methods Flow cytometry and laser confocal microscopy were used to identify hMSH 2 that ectopically expressed on gas-tric carcinoma cell line 803.An anti-hMSH2 antibody was used to block hMSH2 to evaluate its effects on the cytotoxicity of Vγ9δ2 T cells and their cytokines secretion .Subcellular distribution of hMSH 2 in gastric car-cinoma tissues was examined by tissue microarray immunohistochemistry analysis .Results Ectopic mem-brane expression of hMSH 2 was observed on 803 cells at a relatively high level .Vγ9δ2 T cells blocked with specific anti-hMSH2 antibody showed a decreased cytotoxicity and a reduced IFN-γbut an increased TNF-αsecretion.Ectopic expression of hMSH2 was found in various types of gastric carcinoma tissues at different stages.Enhanced expression of hMSH2 was detected in specimens collected from patients with chronic super-ficial gastritis.Conclusion Ectopically expressed hMSH2 served as a stress-induced endogenous ligand which could promote the cytotoxicity of Vγ9δ2 T cells against gastric carcinoma cells and enhance their IFN-γsecretion.hMSH2 played an essential role in innate anti-gastric carcinoma immunity .
