The Regulative Role of ERK5/Bim Pathway in Hypothermal Stimulation Induced Neonatal Rat’s Cardiomyocytes Injury and Apoptosis
10.3969/j.issn.1000-3614.2014.07.016
- VernacularTitle:细胞外信号调节蛋白激酶5/凋亡调节蛋白信号途径在低温诱导心肌细胞损伤及凋亡中的调控作用
- Author:
Yaosheng WANG
;
Xiaoshu CHENG
;
Kui HONG
;
Zonggui WU
;
Yigang LI
- Publication Type:Journal Article
- Keywords:
Hypothermal stimulation;
Cardiomyocytes;
Extracellular regulated protein kinase-5;
Bcl-2 interacting mediator of cell death
- From:
Chinese Circulation Journal
2014;(7):540-544
- CountryChina
- Language:Chinese
-
Abstract:
Objective: To explore the regulative role of extracellular regulated protein kinase-5 (ERK5)/Bcl-2 interacting mediator of cell death (Bim) pathway in hypothermal stimulation induced neonatal rat’s cardiomyocytes (CMs) damage and apoptosis.
Methods: CMs were cultured for hypothermal stimulation and the speciifc siRNA was used to down-regulate the ERK5 or Bim in CMs. The cell apoptosis was detected by lfow cytometry, protein expression was examined by Western blot analysis, the intracellular Ca2+, reactive oxygen species (ROS) and mitochondrial membrane potential (ΔΨm) were evaluated by lfuorescent labeling and lfow cytometry.
Results: In hypothermal stimulated CMs, ERK5 siRNA could promote Bim protein expression, but Bim siRNA could not inlfuence ERK5, while attenuated p-ERK5 expression. ERK5 siRNA induced higher apoptosis rate, while Bim siRNA could decrease such effect. ERK5 siRNA increased the intracellular Ca2+overloading, ROS activation andΔΨm damage, while Bim siRNA played the role to against those effects in hypothermal stimulated CMs.
Conclusion: Our study revealed that ERK5/Bim pathway played the important regulative roll in hypothermal stimulation induced neonatal rat’s CMs damage and apoptosis.
