Determination of Total Polyphenols in Guhong Injection
- VernacularTitle:谷红注射液中总多酚的含量测定
- Author:
Yikun LUO
;
Yanqiu ZHENG
;
Yu HE
- Publication Type:Journal Article
- Keywords:
Guhong Injection;
total polyphenols;
Folin-Ciocalteu colorimetry
- From:
Journal of Zhejiang Chinese Medical University
2014;(7):889-893
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish determination method for total polyphenols in Guhong Injection, and provide reference for internal quality control of Guhong Injection.[Methods]Using the Folin-Ciocalteu colorimetric method, according to the consistent degree of the maximum absorption wavelengths of hydroxysafflor yel ow A and kaempferol relative to Guhong Injection, we selected the appropriate reference and color reaction condition to content determination.[Rsults]With hydroxysafflor yel ow A as the standard, the content of total polyphenols in 10 batches of samples were 9.94, 9.55, 9.75, 9.67, 9.84, 10.03, 9.81, 9.52, 9.88, 10.09mg·mL-1. The average recovery of total polyphenols was 98.11% and RSD was 1.68%(n=6). [Conclusion]The established Folin-Ciocalteu method is simple, accurate, sensitive, and is reliable for detecting total polyphenols in Guhong Injection.
