Effect of type I or type II collagen on biological characteristics of human chondrocytes
10.3969/j.issn.2095-4344.2014.30.014
- VernacularTitle:Ⅰ、Ⅱ型胶原蛋白对人软骨细胞生物学特性的影响
- Author:
Ping JIANG
;
Peng WEI
;
Mingcai ZHAO
;
Qiong CHEN
;
Zi WANG
- Publication Type:Journal Article
- Keywords:
col agen type I;
col agen type II;
chondrocytes
- From:
Chinese Journal of Tissue Engineering Research
2014;(30):4845-4850
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Experiments have shown that the col agen substrate has the capability of stimulating cartilage generation, but the stimulating role of different types of col agen substrates remains controversial. OBJECTIVE:To investigate the effect of type I and type II col agen on the biological characteristics of human chondrocytes cultured in vitro. METHODS:Human chondrocytes at passage were cultured onto the ordinary culture plates (ordinary plate), type I col agen-coated culture plates (type I plate), and type II col agen-coated culture plates (type II plate). cellgrowth curves were determined by MTT method after cells were cultured for 10 days. By ELISA, PCR, and 1,9-dimethyl methyleneblue technology, type I and type II col agen and glycosaminoglycan contents were quantitatively detected in cartilage cells 28 days after culture. RESULTS AND CONCLUSION:The number of cartilage cells was the highest in type II plate, which was twice of that in type I plate and five times of that in ordinary plate. Cartilage cells in type II plate secreted the least amount of type I col agen, which showed significant differences compared with the ordinary plate (P<0.01) and had no statistical y significant difference with type I plate (P>0.01). Cartilage cells in type II plate secreted the most amount of type II col agen and glycosaminoglycan, showing significant differences compared with the other two plates (P<0.01). The cartilage cells cultured in col agen plates are better than that cultured in ordinary culture plate, type II col agen culture plate is better than type I col agen culture plate in maintaining cellshape, extending the dedifferentiation pattern, and promoting celldifferentiation.
