Effects of Yunnan Baiyao on RANK/RANKL/OPG system in porphyromonas gingivalis induced bone destruction
10.3969/j.issn.1671-8348.2014.21.018
- VernacularTitle:云南白药对牙龈卟啉单胞菌诱导骨破坏中RANK／RANKL／OPG系统的影响
- Author:
Yuhua CHEN
;
Xiaobin REN
;
Fangyong ZHU
;
Li LING
;
Hongbing HE
- Publication Type:Journal Article
- Keywords:
Yunnan Baiyao;
porphyromonas gingivalis;
osteoprotegerin;
ligand of receptor activator of NK-κB;
osteoclast
- From:
Chongqing Medicine
2014;(21):2739-2742
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the effects of Yunnan Baiyao on receptor activator of NF-κB(RANK)/receptor activator of NF-κB ligand(RANKL)/osteoprotegerin(OPG) system by means of animal model that use Porphyromonas gingivalis(Pg) induce cal-varial to induce bone destroy .Methods Seventy-two male Kunming mouses were selected and randomly divided into model group , Yunnan Baiyao treatment group and control group .The animals of model group and Yunnan Baiyao treatment group were inoculated Pg at the midline of the scalp between the ears and the animals of the Yunnan Baiyao treatment group gastric perfusion Yunnan Baiyao after them inoculated Pg .Mouses were killed at 5 ,8 and 14 days after inoculation .For each data point ,killed eight mice(n=8) ,then detected the number of osteoclast of the calvaria and the change of RANKL mRNA ,OPGm RNA express .Results On the 5th ,8th and 14th day the number of osteoclast and the content of RANKL mRNA expression in the model group apparently higher than in the Yunnan Baiyao treatment group(P<0 .05) ,two groups also apparently higher than in the control group(P<0 .05) .A-bout OPG mRNA expression on the 5th ,8th and 14th day in the Yunnan Baiyao treatment group apparently higher than in the con-trol group and model group(P<0 .05) ,but on the 5th day the model group lower than the control group(P<0 .05) and on the 8th , 14 day the model group higher than the control group(P<0 .05) .Conclusion Yunnan Baiyao can cut regulation RANKL mRNA expression and raise regulation OPG mRNA expression to inhibit bone destruction and improve bone repair and inhibiting osteoclast product so as to inhibit bone destroy and improve bone repair .
