Effect of theaflavin on LPS-induced inflammatory injury of rat airway epithelial cell and possible mechanism
10.3969/j.issn.1671-8348.2014.21.023
- VernacularTitle:茶黄素在大鼠气道上皮细胞炎症损伤中的作用及可能机制
- Author:
Xiufang WEN
;
Xia CHEN
;
Haiqiao WU
;
Xiangdong ZHOU
- Publication Type:Journal Article
- Keywords:
tumor necrosis factor-alpha;
interleukin-6;
lipopolysaccharide;
theaflavin;
TLR4 mRNA
- From:
Chongqing Medicine
2014;(21):2756-2758
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the influence of theaflavin on the expressions of TLR4 and release of TNF-αand IL-6 in primari-ly cultured rat airway epithelial cells .Methods Lipopolysacchride(LPS) was adopted to establish the in vitro inflammatory injury model of rat airway epithelial cell ,the TNF-αand IL-6 secreted by the airway epithelial cells and the TLR4 protein level in the air-way epithelial cells were detected with ELISA and the expressions of TLR4 mRNA was detected with RT-PCR .Results LPS sig-nificantly induced the airway epithelial cells to secrete TNF-αand IL-6 ,and enhanced the TLR4 mRNA and TLR4 protein expres-sion(P<0 .01) .Theaflavin could inhibit LPS induced TNF-αand IL-6 secretion and TLR4 gene expression(P<0 .05) ,which was related with the theaflavin concentration (P<0 .05) .Conclusion In rat airway epithelial cell culture ,the anti-inflammatory effect of theaflavin may be decrease the release of inflammatory cytokines via inhibiting TLR 4 gene expression and LPS/TLR4 signal trans-duction pathway .
