The impact of celastrol on cognitive function and the expression of Cdk5, p25 and p35 in hippocampus in APPswe/PS1dE9 double transgenic mouse after partial hepatectomy
- VernacularTitle:雷公藤红素对APPswe/PS1dE9双转基因阿尔茨海默病模型小鼠肝叶部分切除术后认知功能及海马内Cdk5、p25和p35表达的影响
- Publication Type:Journal Article
- Keywords: Celastrol; Cdk5; p25; p35; Alzheimers disease; Surgical trauma
- From: The Journal of Clinical Anesthesiology 2014;(7):693-696
- CountryChina
- Language:Chinese
- Abstract: Objective To investigate the impact of celastrol on cognitive function and the ex-pression of Cdk5/p25 in hippocampus in APPswe/PS1dE9 double transgenic mouse after partial hepa-tectomy.Methods Three-month-old APPswe/PS1dE9 double transgenic mice (n=96)were randomly divided into surgery group (partial hepatectomy,group S),celastrol group (celastrol and partial hepa-tectomy,group C)and DMSO group (DMSO and partial hepatectomy,group O).Eight mice were se-lected randomly in each group and were Morris-water maze trained for 5 continuous days.Their learn-ing and memory abilities were evaluated at 1,3,7 and 14 d after the surgery,respectively.For the remaining mice in each group,the hippocampus were collected and the changes of Cdk5,p35 and p25 in hippocampus were measured by western blot at the time 1,3,7,14 d after partial hepatectomy. Six mice were killed at each time for data collection.Results The average escape latency of group C was significantly shorter than those of groups S and O at 3,7 and 14 d after partial hepatectomy (P <0.05).The percentages of time in target quadrant of groups S and O decreased significantly than that in group C (P <0.05).Western blot showed the expression of Cdk5 in group C was significantly low-er than that in groups S and O at 3,7 and 14 d after partial hepatectomy (P <0.05),and the same re-sult was also found in the expression of p25 at 1,3,7 and 14 d after partial hepatectomy (P <0.05). There was no significant difference in the expression of p35 between each group.Conclusion Celas-trol can improve the learning and memory ability in APPswe/PS1dE9 the double transgenic mouse, with the mechanism may be related to the decrease of Cdk5 and p25 in hippocampus.