Purification of human nasal epithelial cells by using immunomagnetic beads
10.3969/j.issn.1671-8348.2014.20.024
- VernacularTitle:免疫磁珠纯化人鼻黏膜上皮细胞的实验研究
- Author:
Zhenzhen CHEN
;
Xin GONG
;
Yang LU
;
Shouying DU
;
Chengbo CAI
;
Kexin SHANG
- Publication Type:Journal Article
- Keywords:
nasal mucosa;
epithelial cells;
primary culture;
immunomagnetic beads
- From:
Chongqing Medicine
2014;(20):2609-2612
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a high purity primary culture methods of human nasal epithelial cells (HNEC) in vitro and to provide a successful primary culture model for evaluation experiments of the nasal preparation .Methods Primary culture of human nasal epithelial cells were performed with enzymatic dissociation of isolated tissue and cultured in serum-free medium .HNEC were separated through magnetic field by immunomagnetic beads .We determined the purity of the separated cells by light microscopy and flow cytometry .The morphology of HNEC was observed with a scanning electron microscope .Results Under an inverted phase microscope ,the cells morphology was paving stone shaped .Under the scanning electron microscopy ,abundant microvilli and cilia differentiation were observed .Flow cytometry showed the epithelial cells accounted for 99% .Conclusion The highly purified HNEC can be directly isolated by the magnetic cell sorting system .The cell model can be used for the basic research of nasal cavity preparation .
