Treatment of aminoguanidine in retina of diabetic of rats with selective inhibits induced nitric oxide synthase
10.3969/j.issn.1671-8348.2014.19.015
- VernacularTitle:氨基胍高选择性抑制iNOS在糖尿病大鼠视网膜中的研究
- Author:
Dawei LUO
;
Haidong ZOU
;
Kun LIU
;
Zhi ZHENG
;
Xiaodong SUN
;
Xun XU
;
Bijun ZHU
- Publication Type:Journal Article
- Keywords:
diabetes mellitus;
aminoguanidine;
nitric oxide synthase
- From:
Chongqing Medicine
2014;(19):2440-2442
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the treatment and mechanism of aminoguanidine in retina of diabetic of rats .Methods To-tal 60 rats were divided into control group(n=20) ,diabetic group(n=20) and aminoguanidine treatment group(n=20)which would be treated by aminoguanidine for 14 days .Then the eye tissue of rats were took after 14 days administration for pathological obser-vation(HE staining) ,and the induced nitric oxide synthase(iNOS) ,endothelial nitric oxide synthase(eNOS) ,nerve type of nitric ox-ide synthase(nNOS) level and the expression of differences content and expression were investigated by ELISA ,Western blot and PT-PCR .Results HE staining showed that retinal tissue defects decreased and neuronal cells of rats in aminoguanidine treatment group were increased and significant (P<0 .05) compared rats in diabetic group .The iNOS content and expression of rats in amin-oguanidine treatment group were lower than diabetic group by ELISA ,Western blot and PT-PCR ,it was significantly difference (P<0 .05) and without significant difference between the normal group and diabetic group (P> 0 .05) .Compared with diabetes group ,iNOS ,eNOS ,nNOS protein expression in the rat retina in aminoguanidine treatment group were reduced (P< 0 .05) ,and without significant difference between the normal group and aminoguanidine treatment group (P>0 .05) .The iNOS mRNA expres-sion was lower than that of eNOS mRNA and nNOS mRNA in aminoguanidine treatment group .Conclusion Aminoguanidine can improve retinal tissue of diabetic rats with lesions ,the pathways may be selectively inhibit inducible nitric oxide synthase activity of iNOS .
