Influence of beryllium ions on genotype of Porphyromonas gingivalis fimbriae gene cluster
10.3969/j.issn.2095-4344.2014.30.010
- VernacularTitle:铍对牙龈卟啉单胞菌菌毛基因型的影响
- Author:
Rui HUANG
;
Qingyan LI
;
Hua LIN
;
Wei GUO
;
Ning GAO
- Publication Type:Journal Article
- Keywords:
beryl ium;
Porphyromonas gingivalis;
fimbriae,bacterial;
pathogenicity
- From:
Chinese Journal of Tissue Engineering Research
2014;(30):4816-4822
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:In oral warm and moisture circumstance, al oy which contains Be is easily to be eroded to release Be2+. But there is stil no research focusing on beryl ium influence on genotype of Porphyromonas gingivalis fimbriae gene cluster. OBJECTIVE:To investigate Be 2+effect on genotype of Porphyromonas gingivalis fimbriae gene cluster. METHODS:The revived Porphyromonas gingivalis was resuscitated for 48 hours in the anaerobic culture medium with different concentration of Be 2+(10×10-6, 5×10-6, 1.25×10-6). Through PCR amplification and sequencing, we investigated the effects of Be2+RESULTS AND CONCLUSION:(1) When Be on genotypes of Porphyromonas gingivalis fimbriae gene cluster. 2+concentration was 5×10-6, we found the peak of 217 and 257 sites on DNA sequence expressing G/A overlap peak, different from G single peak of the other groups, suggesting the suspicious bases changes, part of the single base G mutated into A. (2) On al concentrations, we found a base group composed of seven A bases was inserted into the 101 site of DNA sequence. Up to now, there is no direct contacts of the mutations occurring to Be2+concentration. Changes of gene may lead to the shifting of the reading frame, the abnormal synthesis of proteins, the change of Porphyromonas gingivalis fimA gene toxicity, and lastly the unbalance of the micro-ecological environment.
