Effects of quercetin in combination with bortezomib or lenalidomide on inhibition of proliferation of HL-60 cells
10.3969/j.issn.1006-5725.2014.14.004
- VernacularTitle:槲皮素联合硼替佐米或雷利度胺对抑制HL-60细胞增殖的影响
- Author:
Jie XIAO
;
Guomin NIU
;
Songmei YIN
;
Shuangfeng XIE
;
Yiqing LI
;
Danian NIE
;
Liping MA
;
Xiuju WANG
;
Yudan WU
- Publication Type:Journal Article
- Keywords:
Leukemia;
Quercetin;
Bortezomib;
Lenalidomide
- From:
The Journal of Practical Medicine
2014;(14):2196-2199
- CountryChina
- Language:Chinese
-
Abstract:
Objective Our preliminary study demonstrates that quercetin can inhibit the proliferation of HL-60 cells. This sudy aimed to find some drugs which could have synergistic effects with quercetin on apoptosis of HL-60 cells. Methods HL-60 cells were cultured with bortezomib at different concentrations (1, 2, 4, 8, 16, and 32μmol/L) alone or combined with quercetin at different concentrations for 48 h. HL-60 cells were cultured with lenalidomide at different concentrations (5, 10, 20, 40, 80, 160, and 320 μmol/L) alone or in combination with quercetin at different concentrations for 48 h. The CCK-8 assay was used to determine the effects on proliferation of HL-60 cells. Results Bortezomib significantly inhibited the proliferation of HL-60 cells (P<0.01). IC50 of quercetin was 49.24μmol/L after cells treated by quercetin combined with bortezomib, which was 13.44μmol/L lower than that treated by quercetin alone. Isobolographic analysis revealed the two drugs had synergistic effect. The results of cell viability of HL-60 cells treated by lenalidomide at lower concentrations (5, 10, 20, 40, and 80μmol/L)were not different from those of the control group (P > 0.05). The results of cell viability of HL-60 cells treated by lenalidomide at higher concentrations (160 and 320μmol/L) were lower than those of the control group (P<0.05). IC50 of quercetin after cells treated by quercetin combined with bortezomib was not different from that treated by quercetin alone. Isobolographic analysis revealed the two drugs had no synergistic effect. Conclusions Bortezomib can inhibit the proliferation of HL-60 cells and it has a synergistic effect with quercetin on HL-60 cells. Lenalidomide has a weaker role in inhibition of the proliferation of HL-60 cells, and it has no synergistic effect with quercetin on HL-60 cells.
