Effect of DNA oxidative damage on micronucleus frequency in peripheral blood lymphocytes
10.3969/j.issn.1673-4130.2014.14.004
- VernacularTitle:DNA 氧化损伤对人外周血淋巴细胞微核率的影响
- Author:
Changye HUI
;
Yan GUO
;
Chaoxian GAO
;
Dianpeng WANG
;
Wen ZHANG
;
Xinyue YANG
;
Zhimin LI
- Publication Type:Journal Article
- Keywords:
lymphocyte;
DNA oxidative damage;
micronucleus
- From:
International Journal of Laboratory Medicine
2014;(14):1823-1824
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the effect of DNA damage induced by H2 O2 on the micronucleus frequency in lymphocytes. Methods Resting lymphocytes were treated with different levels of H2 O2 (10,50,100,1 000 μmol/L).1 000 μmol/L H2 O2 was added into mitogen-stimulated lymphocyte cultures at different time intervals.Then micronucleus rate was examined by the conven-tional culture method.Results There was no significant change of the micronucleus frequency in the experimental groups.Conclu-sion H2 O2 could induce lymphocyte DNA damage rapidly,but exerts no effect on the formation of micronuclei,which may be relat-ed to the type of DNA damage and rapid DNA repair.
