Research on DNA microarray chip method for detecting drug resistance of Mycobacterium tuberculosis
10.3969/j.issn.1673-4130.2014.14.040
- VernacularTitle:DNA 微阵列芯片法用于检测结核分枝杆菌耐药性的研究
- Author:
Yaqin LIU
;
Zhenbin YANG
;
Dongxia FENG
;
Haiying WANG
- Publication Type:Journal Article
- Keywords:
DNA microarray;
Mycobacterium tuberculosis;
nucleotide hybridization;
drug resistance;
mutation
- From:
International Journal of Laboratory Medicine
2014;(14):1910-1913
- CountryChina
- Language:Chinese
-
Abstract:
Objective To evaluate the feasibility of the DNA microarray method used in detecting the drug resistance of Myco-bacterium tuberculosis by comparing the traditional proportion method and the DNA microarray method for detecting the drug re-sistance of Mycobacterium tuberculosis.Methods 54 strains of Mycobacterium tuberculosis isolated from clinical specimens in our hospital from January 2012 to March 2013 were randomly extracted and their resistance to isonicotinic acid hydrazide (INH)and rifampicin(RFP)was detected by the DNA microarray method and the proportion method.The detection results were performed the comparative analysis.Results With the proportion method as the golden standard,the coincidence rates of the DNA microarray method for detecting the Mycobacterium tuberculosis resistance to INH and RFP were 75% and 91.0% respectively.Conclusion The DNA microarray technique is suitable for the rapid screening of clinical first-line drug resistant Mycobacterium tuberculosis.
