Effect of miR-19 on migration ability of lung adenocarcinoma cell line A549-Luc
10.3969/j.issn.1006-5725.2014.13.005
- VernacularTitle:miR-19对肺腺癌A549细胞株迁移功能的影响
- Author:
Wen YAN
;
Wen JIN
;
Ang WANG
;
Weishu SONG
- Publication Type:Journal Article
- Keywords:
Lung adenocarcinoma;
miR-19;
Migration
- From:
The Journal of Practical Medicine
2014;(13):2031-2034
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of miR-19 on the migration ability of lung adenocarcinoma cell line A549. Methods The expressions of miR-19 in lung epithelial cell line BEAS-2B and lung adenocarcinoma cell line A549-Luc were detected by qRT-PCR.The A549-Luc cell line which over-expressed miR-19 was established. The expression levels of miR-19 in A549/RFP+/H2B and A549/RFP+/m19 were identified by qRT-PCR. The morphology of A549/RFP+/m19 was observed,and the migration ability of A549/RFP+/m19 was detected by transwell migration assay. Results The expression levels of miR-19 differed significantly between BEAS-2B cells and A549-Luc cells (t = -20.954, P < 0.001). The lung adenocarcinoma cell line A549/RFP+/m19 which over-expressed miR-19 was successfully established. Changes in A549/RFP+/m19 cell morphology were found. As compared with A549/RFP+/H2B cells, A549/RFP+/m19 had an increased migration ability (P <0.01). Conclusions miR-19 enhances the migration ability of lung adenocarcinoma A549-Luc cells.