Effects of preconditioning with sodium butyrate on myocardial ischemia/reperfusion injury in rats

VernacularTitle:丁酸钠预处理对大鼠心肌缺血／再灌注损伤的影响
Author: Hong JIANG ; Chunfeng YI ; Yuanhong LI ; Xiaorong HU ; Changwu XU
Publication Type:Journal Article
Keywords: Myocardial ischemia; Sodium butyrate; Reperfusion; High mobility group box 1 protein
From: The Journal of Practical Medicine 2014;(13):2034-2037
CountryChina
Language:Chinese
Abstract: Objective To investigate the effect of preconditioning with sodium butyrate on myocardial I/R injury. Methods Anesthetized rats were treated with sodium butyrate (100 or 300 mg/kg, i.p.) 30 mins before ischemia, and then subjected to ischemia for 30 min followed by reperfusion for 4 h. LDH, CK, TNF-α, IL-6, HMGB1, infarct size, MDA and SOD activity were measured. The infracted size was tested by TTC assay; The expression of HMGB1 was observed by western blot. Results After 4 h reperfusion, pretreatment of sodium butyrate (300 mg/kg) could significantly reduce the infarct size and the levels of LDH and CK (P<0.05)comparing to the control group; inhibit the increase of the MDA level and the decrease of the SOD level(P<0.05), also inhibit the expression of TNF-α, IL-6 and HMGB1 (all P < 0.05) induced by I/R. Conclusion Preconditioning of sodium butyrate can attenuate myocardial I/R injury by inhibiting inflammation response.