Effect of simulated microgravity on proliferation and differentiation of the human megakaryocyte cell
10.3969/j.issn.1006-5725.2014.12.005
- VernacularTitle:模拟微重力对巨核细胞增殖和分化的影响
- Author:
Chunyan YUE
;
Xinru MAO
;
Lei ZHENG
;
Ya GAO
;
Yangmin ZHU
;
Bin WU
;
Jiaqiong HONG
;
Baohong PING
- Publication Type:Journal Article
- Keywords:
Simulated microgravity;
Megakaryocyte;
c-mpl;
GATA-1;
NF-E2;
RUNX1
- From:
The Journal of Practical Medicine
2014;(12):1867-1870
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of simulated microgravity on the proliferation and differentiation of the human megakaryocyte cells in vitro. Methods The fourth generation rotating cell culture system (RCCS-4) was used to generate the simulated microgravity environment. The cell viability was assessed by trypan blue staining method. The proliferation of cells was assessed by cell counting method and CCK8 method. The CD41+/CD61+ cells rate and the cells cycle were detected by flow cytometry. The expression levels of thrombopoietin receptor (c-mpl) and transcription factors were detected with RT-PCR. Results After 24, 48, 72 h, culture under simulated microgravity resulted in a significant decrease in the cell number , proliferative activity, cells in the G2/M phase and levels of c-mpl mRNA expression in comparison with that under the normal gravity (P < 0.05). After 48 h and 72 h culture, CD41+/CD61+ cells ratio decreased and RUNX-1 mRNA expression was down-regulated in cells of the group SMG compared with that of the group NG (P < 0.05). Conclusion Microgravity can inhibit the proliferation and differentiation of human megakaryocyte cells in vitro. The mechanism may be that TPO/c-mpl pathway was inhibited by down regulating the expression of c-mpl which transcriptional inhibition lead to.
