Effects of tyrosine kinase Src during C.albicans infection in murine macrophage
10.3969/j.issn.1006-5725.2014.11.007
- VernacularTitle:酪氨酸激酶Src在白色念珠菌感染中的机制研究
- Author:
Shuna CUI
;
Jing QIAN
;
Ping BU
- Publication Type:Journal Article
- Keywords:
Candidiasis;
Src kinase;
C. albicans;
Macrophage
- From:
The Journal of Practical Medicine
2014;(11):1702-1704
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the role of tyrosine kinase Src in a murine C.albicans infection model. Methods Observed cell proliferation by alarmarblue assay at 2, 24 and 48 h after Src inhibitor PP2 treatment. Phagocytosis was determined by a fluorometric assay. Cytokine TNF-αand IL-10 production was detected by ELISA. Results The 0~33.3 μmol/L PP2 had no effect on cell proliferation after PP2 treatment for 2 h. When the PP2 treatment extended to 24 or 48 h, PP2 (11.1, 33.3μmol/L) showed significant inhibition on cell proliferation with 78%, 9%, and 54%,13%, respectively. At 48 h after 11.1μmol/L PP2 treatment, the internalization of C.albicans in macrophage is significantly inhibited, contributing to the inhibition of cell proliferation. However, the 11.1 and 33.3μmol/L PP2 significantly inhibited the cytokine TNF-αand IL-10 production during C.albicans infection (P<0.01). Conclusion Src kinase played an important role during C.albicans infection, especially for the cytokine TNF-αand IL-10 production.
