Study on apoptosis-inducing mechanism of dihydromyricetin on human liver cancer HepG2
10.3969/j.issn.1006-5725.2014.11.010
- VernacularTitle:二氢杨梅素诱导肝癌细胞株HepG2凋亡途径的探讨
- Author:
Junhua CEN
- Publication Type:Journal Article
- Keywords:
Dihydromyricetin;
HepG2;
Liver Cancer Cell;
Proliferation;
Apoptosis
- From:
The Journal of Practical Medicine
2014;(11):1712-1715
- CountryChina
- Language:Chinese
-
Abstract:
Objective To discuss the proliferation inhibition, apoptosis-inducing effects and the mechanism of dihydromyricetin on human liver cancer HepG2. Methods The effects of dihydromyricetin on cell death rate and proliferation in light of MTT test were examined and IC50 was calculated. The effects of dihydromyricetin on liver cancer HepG2 in different cell cycles were observed and analyzed in light of HE fluorescent staining method and Annexin V-PI flow cytometry (FCM). The protein expressions of p34cdc-2、CyclinB1、Bcl-2 and PARP which were related to the cell cycle and apoptosis before and after the use of dihydromyricetin were determined in light of Western blotting. Results The death rate of liver cancer HepG2 were increased with the increasing of dihydromyricetin concentration. The IC50 read (35.22 ± 1.56)μmol/L and displayed dose-response relationship. Dihydromyricetin had significant impacts on liver cancer HepG2 in different cell cycles, the proportion of G2/M cells rose dramatically and that of S cells lowered sharply and that of G0/G1 cell decreased exponentially.The difference was of statistical significance compared with the control group (P < 0.05). Conclusion Dihydromyricetin has distinct medical effects on inhibiting the growth and inducing the apoptosis of liver cancer HepG2. The mechanism is to activate Caspase-3,thus engaged in apoptosis in light of signal transduction of the mitochondrion.
