The preliminary analysis of peripheral blood circulating microRNA expression profile in patients with ischemic and hemorrhagic stroke
10.3969/j.issn.1006-5725.2014.11.021
- VernacularTitle:脑梗死和脑出血患者外周血中循环microRNA表达谱差异的初步分析
- Author:
Wuying LI
;
Jun JIN
;
Jian CHEN
;
Yang GUO
;
Jun TANG
;
Sheng TAN
- Publication Type:Journal Article
- Keywords:
Brain infarction;
Cerebral hemorrhage;
Circulating microRNA;
MicroRNA array
- From:
The Journal of Practical Medicine
2014;(11):1750-1753
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the peripheral blood circulating microRNA expression profile of ischemic and hemorrhagic stroke patients by using microRNA array. Methods The whole blood of 3 patients with acute ischemic stroke, 3 patients with cerebral hemorrhage and 3 healthy volunteers were collected for whole blood RNA through Norgen exosome isolation kit, and then underwent quality and density measurement through PCR. The miRNAs expression profile was studied through μParaflorTM microRNA. All the data were analyzed by associated software and statistics methods. Results 8 miRNAs associated with cerebral stroke in ischemia stroke patients and cerebral hemorrhage patients were discovered, and showed significant difference (P<0.05). 11 differential expression miRNAs were found between ischemia stroke patients and control group, and 24 were found between hemorrhage patients and control group. However, after up-regulation, no significant difference was found (P > 0.05). Conclusions Different expression profiles of miRNA between ischemic stroke patients and hemorrhagic stroke patients may provide insights into their roles in the pathogenesis and progression of stroke. The study of miRNA can contributes to elucidate the epigenetics molecular mechanism of acute stroke.
