Optimal concentration of modified platelet-rich plasma to promote the proliferation of dental pulp stem cells from deciduous teeth
10.3969/j.issn.2095-4344.2014.28.015
- VernacularTitle:改良富血小板血浆促进乳牙牙髓干细胞增殖的最佳浓度
- Author:
Jun WEN
;
Buling WU
;
Jianmin DUAN
;
Hongtao LI
;
Sihan LI
;
Xin LI
- Publication Type:Journal Article
- Keywords:
platelet-rich plasma;
tooth;
deciduous;
dental pulp;
serum albumin;
bovine
- From:
Chinese Journal of Tissue Engineering Research
2014;(28):4517-4523
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Previous experiments have shown that modified platelet-rich plasma activated by liquid nitrogen freezing and thawing can promote the proliferation of human bone marrow mesenchymal stem cells and dental pulp stem cells in a concentration-dependent manner. OBJECTIVE:To investigate the effect of modified platelet-rich plasma at different concentrations on the proliferation of dental pulp stem cells from human exfoliated deciduous teeth. METHODS:Platelets were selected and harvested by automatic blood cellanalyzer, and then activated by liquid nitrogen freezing and thawing.α-MEM served as basal medium. Different concentrations of modified platelet-rich plasma (2%, 5%, 10%, 20%) or 10%fetal bovine serum were added, respectively. The difference in cellproliferation was observed. RESULTS AND CONCLUSION:Modified platelet-rich plasma at different concentrations could promote the proliferation of dental pulp stem cells from deciduous teeth. The effects of 2%modified platelet-rich plasma and 10%fetal bovine serum on promoting the proliferation of dental pulp stem cells from deciduous teeth were similar. These results indicated that 2%modified platelet-rich plasma could promote the proliferation of dental pulp stem cells from deciduous teeth, and substitute for fetal bovine serum in the amplification of dental pulp stem cells from deciduous teeth in vitro.
