Human cytomegalovirus detection in hematopoietic stem cell transplantation:value of fluorescence quantitative PCR in the early diagnosis
10.3969/j.issn.2095-4344.2014.28.022
- VernacularTitle:造血干细胞移植中人巨细胞病毒检测：荧光定量聚合酶链反应的早期诊断价值
- Author:
Chuan LIU
;
Yeqing ZOU
;
Qingzhi SHI
- Publication Type:Journal Article
- Keywords:
hematopoietic stem celltransplantation;
cytomegalovirus;
cytomegalovirus infections;
polymerase chain reaction
- From:
Chinese Journal of Tissue Engineering Research
2014;(28):4563-4567
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:To improve the survival rate of transplanted hematopoietic stem cells, dynamic monitoring of plasma content of human cytomegalovirus (HCMV) and rapid screening of early active HCMV infection in hematopoietic stem celltranplantation recipients, thus to guide the clinical medication, is preferred. OBJECTIVE:To evaluate the usefulness of fluorescence quantitative PCR assay for early detection of HCMV activation after hematopoietic stem celltransplantation. METHODS:Real-time fluorescence quantitative PCR assay was applied for HCMV monitoring in 656 blood samples from 41 hematopoietic stem celltranplantation recipients and 60 control blood samples. RESULTS AND CONCLUSION:In 656 blood samples, 96 samples were positive, and the HCMV DNA copies ranged from 5.0×102 to 1.0×107 IU/mL. Timely initiation of therapy resulted in the rapid clearance of DNA-viraemia but it recurrenced in short time by drug-resistent. Two cases from 12 postive recipients died from HCMV infection. The quantitative detection of HCMV DNA by real-time PCR is a rapid method for monitoring HCMV infection and the early diagnosis in patients after hematopoietic stem celltransplantation.
