In vitro expansion of human mesenchymal stem cells in animal serum-free media
10.3969/j.issn.2095-4344.2014.28.026
- VernacularTitle:无动物源性成分培养基体外扩增的间充质干细胞
- Author:
Di WU
;
Xiaoyun WU
;
Yan WU
- Publication Type:Journal Article
- Keywords:
mesenchymal stem cells;
culture media;
serum;
platelet-rich plasma
- From:
Chinese Journal of Tissue Engineering Research
2014;(28):4583-4587
- CountryChina
- Language:Chinese
-
Abstract:
BACKGROUND:Mesenchymal stem cells have been an important source of seed cells for tissue engineering, regenerative medicine and immunosuppressive therapy areas. The large expansion is the key to the clinical application of mesenchymal stem cells. OBJECTIVE:To summarize the research progress in the in vitro expansion of human mesenchymal stem cells in animal serum-free media. METHODS:A computer-based online retrieval of CNKI and PubMed databases was performed to search papers published between 2004 and 2014 using the key words of“mesenchymal (stromal) stem cells, animal serum-free media, humanized media, human serum, umbilical cord blood serum, platelet rich plasma, platelet lysate, defined medium”in English and Chinese, respectively. Final y, 41 articles were retained. RESULTS AND CONCLUSION:Human serum, umbilical cord blood serum, platelet rich plasma, platelet lysate and defined medium can replace fetal bovine serum for large-scale expansion of mesenchymal stem cells in vitro. These media or additives have their advantages and disadvantages;therefore, it is urgent to guarantee the functional effectiveness and transplantation safety before large-scale, systematic clinical application of mesenchymal stem cells.
