Effect of GLP-1 on the expression of PDX-1 gene in islet beta cells of type 2 diabetes rats
10.3969/j.issn.1006-5725.2014.11.006
- VernacularTitle:2型糖尿病大鼠胰岛β细胞PDX-1基因表达及GLP-1的干预作用
- Author:
Meifen WU
;
Xiaoming CHEN
;
Ge WU
;
Kunjie ZHENG
;
Haiyan PAN
;
Shuo FANG
- Publication Type:Journal Article
- Keywords:
Diabetes mellitus,type 2;
Pancreatic duodenal homeobox-1;
Glucagon-like peptide-1;
Liraglutide
- From:
The Journal of Practical Medicine
2014;(11):1699-1701
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of liraglutide, an analogue of glucagon like peptide-1 (GLP-1), on the expression of PDX-1 gene in the islet beta cells of type 2 diabetes rats. Methods Sixty male SD rats were divided into 3 groups:normal control group, diabetic model group and GLP-1 group. Normal control group was given normal diet and diabetic model group and GLP-1 group were given high fat diet. After the eighth weekend, the high fat diet group was intraperitoneally injected with streptozotocin (STZ) 30 mg/(kg·bw), and the GLP-1 group was subcutaneously injected with liraglutide 200μg/kg with 2 times per day for 8 weeks. The other groups were treated with normal saline by subcutaneous injection. Real-time fluorescent quantitative reverse transcription polymerase chain reaction (Real-Time RT-PCR) was used to detect the expression of PDX-1 gene in rat pancreatic islet cells. Results The expressions of PDX-1 mRNA in the GLP-1 group and untreated group were significantly lower (P <0.001) than that in the normal control group, but significantly higher than that after GLP-1treatment (P < 0.001). Conclusion GLP-1 can upregulate the expression of PDX-1 gene in the islet beta cells of type 2 diabetes rats.
