Investigation on Enterobacteriaceae producing KPC carbapenemase in Huizhou city of Guangdong ;province
10.3760/cma.j.issn.1008-6706.2013.s2.001
- VernacularTitle:广东省惠州市肠杆菌科细菌产KPC 碳青霉烯酶的调查研究
- Author:
Haiyan CAO
;
Lu LIU
;
Xiaoxia TONG
;
Ling WEI
- Publication Type:Journal Article
- Keywords:
Enterobacteriaceae;
Drug resistance,microbial
- From:
Chinese Journal of Primary Medicine and Pharmacy
2013;20(z2):1-3
- CountryChina
- Language:Chinese
-
Abstract:
Objective To survey the status of clinical isolates multi-drug resistant Enterobacteriaceae produ-cing KPC carbapenemase in Huizhou city of Guangdong Province .Methods 81 strains of multi-drug resistant Enter-obacteriaceae were collectded from 2009 to 2011 in Huizhou region.Modified Hodge test(MHT) was used to screen stains which may produce carbapenemase , modified three-dimensional test was used to detect ESBLs , AmpC and MBLs.Polymerase chain reaction (PCR) and gene sequencing methods were performed to confirm KPC genotypes . Results Of 81 strains multi-drug resistant enterobacteriaceae ,13 of which had passitive results were screened by modified Hodge test ,including 7 strains of Enterobacter cloacae ,4 strains of Klebsiella pneumonia and 2 strains of Escherichia coli;By modified three-dimensional test ,9 strains produced MBLs ,2 strains produced ESBLs ,1 strain pro-duced AmpC ,1 strain produced ESBLs and AmpC at the same time .KPC was amplified from a strain of Klebsiella pneumonia which produce ESBLs by PCR ,KPC-2 genetype was identified by gene sequencing .Conclusion Klebsiel-la pneumonia with KPC–2 carbapenemase has emerged in huizhou city ,but the production of metallo-beta-lactamase is the main cause that Enterobacteriaceae resist to carbapenemase drugs .
